Lablisa™ Human Ceramide-1-phosphate(C1P) ELISA Kit
Quality guarantee period：for 12 months
Coated ELISA plate
12-Well * 8 Tubes
Washing concentrate (30X)
Chromogen solution A
Seal plate membrane
Chromogen solution B
Antibodies labeled with biotin
The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma,tissue homogenates,cell lysates,cell culture supernates and other biological fluids.
For unopened kit: All the reagents should be kept according to the labels on vials, Washing concentrate (30X) and the Stop Solution should be stored at 4℃ upon receipt while the others should be at -20℃
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
ASSAY PROCEDURE SUMMARY
1. Prepare all reagents, samples and standards;
2. Add prepared samples, standards and ELISA solutions. Let them react for 60 minutes at 37 ℃.
3. Wash the plate five times. Add Chromogen solution A and B. Incubate for 10 minutes at 37 ℃; for color development.
4. Add stop solution
5. Read the OD value within 10minutes.