Lablisa™ Human Ceramide-1-phosphate(C1P) ELISA Kit
Product Information
- Description
- More Infomation
This kit uses enzyme-linked immune sorbent assay (ELISA) based on the Biotin double antibody sandwich technology to assay the Human Ceramide-1-phosphate (C1P). Add Ceramide-1-phosphate(C1P)to the wells, which are pre-coated with Ceramide-1-phosphate(C1P)monoclonal antibody and then incubate. After that, add anti C1P antibodies labeled with biotin to unite with streptavidin-HRP, which forms immune complex. Remove unbound enzymes after incubation and washing. Add substrate A and B. Then the solution will turn blue and change into yellow with the effect of acid. The shades of solution and the concentration of Human Ceramide-1-phosphate (C1P) are positively correlated.
Method：sandwich
Detection range：1pg/mL→100pg/mL
Sensitivity：0.14pg/mL
Assay length：1-4.5Hours
Detection Wavelengt：450nm
Research Area：Immunology
Quality guarantee period：for 12 months
Introduction
Reagents | Quantity | Reagents | Quantity |
Coated ELISA plate | 12-Well * 8 Tubes | Washing concentrate (30X) | 20ml |
Standard dilution | 3ml | Instruction | 1 |
Chromogen solution A | 6ml | Seal plate membrane | 2 |
Chromogen solution B | 6ml | Hermetic bag | 1 |
Streptavidin-HRP | 6ml | Stop solution | 6ml |
Standard solution | 0.5ml | Antibodies labeled with biotin | 1ml |
INTENDED USE
The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma,tissue homogenates,cell lysates,cell culture supernates and other biological fluids.
STORAGE
For unopened kit: All the reagents should be kept according to the labels on vials, Washing concentrate (30X) and the Stop Solution should be stored at 4℃ upon receipt while the others should be at -20℃
PRECISION
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<8%
Inter-Assay: CV<10%
ASSAY PROCEDURE SUMMARY
1. Prepare all reagents, samples and standards;
2. Add prepared samples, standards and ELISA solutions. Let them react for 60 minutes at 37 ℃.
3. Wash the plate five times. Add Chromogen solution A and B. Incubate for 10 minutes at 37 ℃; for color development.
4. Add stop solution
5. Read the OD value within 10minutes.
6. Calculate.
