ELISA Kits

Lablisa® Human OB(Obestatin) ELISA Kit

Catalog No.: LAB11051

Competitive

Reactivity	Human
Detection Range	0.79-50ng/mL
Sensitivity	0.47ng/mL

Size:

48T ₹32,550.00 96T ₹42,550.00

Description

This ELISA kit uses the Competitive-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with Human OB. During the reaction, Human OB in the sample or standard competes with a fixed amount of Human OB on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human OB. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human OB in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Technical Specifications

Catalog Number	LAB11051
Reactivity	Human
Detection Range	0.79-50ng/mL
Sensitivity	0.47ng/mL

Product Overview

Product name:	Lablisa® Human OB(Obestatin) ELISA Kit
Reactivity:	Human
Alternative Names:	OB
Assay Type:	Competitive
Sensitivity:	0.47ng/mL
Standard:	50ng/mL
Range:	0.79-50ng/mL
Sample Type:	Serum, Plasma and Tissue homogenate and other biological samples；Sample volume：25μL
Assay Length:	1.5H
Research Area:	Metabolic pathway;Endocrinology;Hormone metabolism;

Standard curve

Concentration (pg/mL)	OF
50	0.238
25	0.361
12.5	0.591
6.25	0.826
3.13	1.187
1.56	1.56
0.78	1.911
0	2.56

Precision

Intra-assay Precision (Precision within an assay) ：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) ：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant Human OB and the recovery rates were calculated by comparing the measured value to the expected amount of Human OB in samples.

Matrix	Recovery range	Average
serum (n=8)	94-103%	98%
EDTA plasma (n=8)	87-97%	92%
Cell culture media (n=8)	92-99%	95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human OB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.