ELISA Kits
Lablisa® Mouse β-CTx(Beta Crosslaps) ELISA Kit
Catalog No.: LAB11352
| Reactivity | Mouse |
| Detection Range | 78.13-5000pg/mL |
| Sensitivity | 46.88pg/mL |
Description
This ELISA kit uses the Competitive-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with Mouse β-CTx. During the reaction, Mouse β-CTx in the sample or standard competes with a fixed amount of Mouse β-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse β-CTx. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Mouse β-CTx in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Technical Specifications
| Catalog Number | LAB11352 |
| Reactivity | Mouse |
| Detection Range | 78.13-5000pg/mL |
| Sensitivity | 46.88pg/mL |
Product Overview
Product name: | Lablisa® Mouse β-CTx(Beta Crosslaps) ELISA Kit |
| Reactivity: | Mouse |
| Alternative Names: | b-CTx, bCTXI, bCTX-I, B-Cr, BCL, Type I Collagen C-Telopeptide-Related Fraction |
| Assay Type: | Competitive |
| Sensitivity: | 46.88pg/mL |
| Standard: | 5000pg/mL |
| Range: | 78.13-5000pg/mL |
| Sample Type: | Serum, Plasma and Tissue homogenate and other biological samples；Sample volume：25μL |
| Assay Length: | 1.5H |
| Research Area: | Signal transduction;Hematology; |
Standard curve
| Concentration (pg/mL) | OF |
|---|---|
| 5000 | 0.205 |
| 2500 | 0.389 |
| 1250 | 0.633 |
| 625 | 0.945 |
| 312.5 | 1.197 |
| 156.25 | 1.484 |
| 78.13 | 2.003 |
| 0 | 2.786 |
Precision
Intra-assay Precision (Precision within an assay) ：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) ：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Mouse β-CTx and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse β-CTx in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum (n=8) | 88-97% | 92% |
| EDTA plasma (n=8) | 87-95% | 91% |
| Cell culture media (n=8) | 87-95% | 90% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse β-CTx and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum (n=8) | 99-107% | 94-102% | 90-98% | 95-104% |
| EDTA plasma (n=8) | 93-103% | 99-106% | 98-107% | 97-107% |
| Cell culture media (n=8) | 91-98% | 94-100% | 94-103% | 94-103% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
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Protocol
For detailed protocol, assay guidance, and technical support, please contact our Customer Support team at
support@labrecon.in
support@labrecon.in
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