ELISA Kits
Lablisa® Rat DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit
Catalog No.: LAB12442
| Reactivity | Rat |
| Detection Range | 31.25-2000pg/mL |
| Sensitivity | 18.75pg/mL |
Description
This ELISA kit uses the Competitive-ELISA principle. The micro-ELISA plate provided in this kit has been pre-coated with Rat DHEA-S. During the reaction, Rat DHEA-S in the sample or standard competes with a fixed amount of Rat DHEA-S on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat DHEA-S. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat DHEA-S in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Technical Specifications
| Catalog Number | LAB12442 |
| Reactivity | Rat |
| Detection Range | 31.25-2000pg/mL |
| Sensitivity | 18.75pg/mL |
Product Overview
Product name: | Lablisa® Rat DHEA-S (Dehydroepiandrosterone Sulfate) ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | Androstenolone sulfate; Prasterone sulfate; Androst-5-en-3β-ol-17-one 3β-sulfate |
| Assay Type: | Competitive |
| Sensitivity: | 18.75pg/mL |
| Standard: | 2000pg/mL |
| Range: | 31.25-2000pg/mL |
| Sample Type: | Serum, Plasma and Tissue homogenate and other biological samples；Sample volume：25μL |
| Assay Length: | 1.5H |
| Research Area: | Endocrinology;Hepatology;Hormone metabolism; |
Standard curve
| Concentration (pg/mL) | OD |
|---|---|
| 2000 | 0.24 |
| 1000 | 0.361 |
| 500 | 0.687 |
| 250 | 0.813 |
| 125 | 1.234 |
| 62.5 | 1.621 |
| 31.25 | 1.998 |
| 0 | 2.464 |
Precision
Intra-assay Precision (Precision within an assay) ：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) ：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Rat DHEA-S and the recovery rates were calculated by comparing the measured value to the expected amount of Rat DHEA-S in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum (n=8) | 94-102% | 99% |
| EDTA plasma (n=8) | 92-98% | 95% |
| Cell culture media (n=8) | 91-99% | 95% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rat DHEA-S and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum (n=8) | 91-97% | 92-100% | 93-101% | 97-107% |
| EDTA plasma (n=8) | 96-104% | 94-103% | 91-99% | 99-106% |
| Cell culture media (n=8) | 95-103% | 89-97% | 95-101% | 93-102% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
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Protocol
For detailed protocol, assay guidance, and technical support, please contact our Customer Support team at
support@labrecon.in
support@labrecon.in
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