Lablisa® Human E-Cad(E-Cadherin) ELISA Kit

Product Information

Lablisa® Human E-Cad(E-Cadherin) ELISA Kit 

Catalog No : LAB1006 | Pack Size : 48T , 96T

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E-Cadherin(E-Cad). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E-Cadherin(E-Cad). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E-Cadherin(E-Cad), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E-Cadherin(E-Cad) in the samples is then determined by comparing the OD of the samples to the standard curve.

Product name:Lablisa® Human E-Cad(E-Cadherin) ELISA Kit
Alternative Names:CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule; Cadherin, Epithelial
Assay Type:Sandwich
Sensitivity:25.1 pg/mL
Standard:4000 pg/mL
Range:62.5-4000 pg/mL
Sample Type:serum, plasma, urine, tissue homogenates and other biological fluids
Assay Length:3.5h
Research Area:CD & Adhesion molecule; Tumor immunity;

Standard curve

Concentration (ng/mL)ODCorrected OD


Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.


Matrices listed below were spiked with certain level of recombinant E-Cad and the recovery rates were calculated by comparing the measured value to the expected amount of E-Cad in samples.

MatrixRecovery rangeAverage
EDTA plasma(n=5)95-107%101%
Heparin plasma(n=5)82-95%88%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E-Cad and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

EDTA plasma(n=5)87-97%86-92%89-103%89-98%
Heparin plasma(n=5)79-85%87-96%82-95%89-103%

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