Lablisa® Human E-Cad(E-Cadherin) ELISA Kit
Product Information
- Description
- More Infomation
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E-Cadherin(E-Cad). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E-Cadherin(E-Cad). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E-Cadherin(E-Cad), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E-Cadherin(E-Cad) in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® Human E-Cad(E-Cadherin) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | CD324; CDH1; Arc1; ECAD; CDH-E; E-CAD; LCAM; UVO; Cadherin 1 Type 1; Uvomorulin; E-Cadherin; Calcium-Dependent Adhesion Protein, Epithelial; Liver Cell Adhesion Molecule; Cadherin, Epithelial |
| Assay Type: | Sandwich |
| Sensitivity: | 25.1 pg/mL |
| Standard: | 4000 pg/mL |
| Range: | 62.5-4000 pg/mL |
| Sample Type: | serum, plasma, urine, tissue homogenates and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | CD & Adhesion molecule; Tumor immunity; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 4000.00 | 2.201 | 2.076 |
| 2000.00 | 1.717 | 1.592 |
| 1000.00 | 1.251 | 1.126 |
| 500.00 | 0.806 | 0.681 |
| 250.00 | 0.539 | 0.414 |
| 125.00 | 0.364 | 0.239 |
| 62.50 | 0.258 | 0.133 |
| 0.00 | 0.125 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant E-Cad and the recovery rates were calculated by comparing the measured value to the expected amount of E-Cad in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 85-97% | 91% |
| EDTA plasma(n=5) | 95-107% | 101% |
| Heparin plasma(n=5) | 82-95% | 88% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E-Cad and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 95-102% | 96-104% | 87-98% | 95-107% |
| EDTA plasma(n=5) | 87-97% | 86-92% | 89-103% | 89-98% |
| Heparin plasma(n=5) | 79-85% | 87-96% | 82-95% | 89-103% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
