Lablisa® Human TIMP1(Tissue Inhibitors Of Metalloproteinase 1) ELISA Kit
Product Information
- Description
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tissue Inhibitors Of Metalloproteinase 1(TIMP1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tissue Inhibitors Of Metalloproteinase 1(TIMP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tissue Inhibitors Of Metalloproteinase 1(TIMP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tissue Inhibitors Of Metalloproteinase 1(TIMP1) in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Human TIMP1(Tissue Inhibitors Of Metalloproteinase 1) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | EPO; CLGI; EPA; HCI; Erythroid Potentiating Activity; Collagenase Inhibitor; Metalloproteinase inhibitor 1; Fibroblast collagenase inhibitor |
| Assay Type: | Sandwich |
| Sensitivity: | 0.059 ng/mL |
| Standard: | 10 ng/mL |
| Sample type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay length: | 3.5h |
| Research Area: | Tumor immunity;Infection immunity;Cardiovascular biology;Hepatology;Hormone metabolism; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 10.00 | 2.058 | 1.990 |
| 5.00 | 1.562 | 1.494 |
| 2.50 | 1.143 | 1.075 |
| 1.25 | 0.797 | 0.729 |
| 0.63 | 0.519 | 0.451 |
| 0.32 | 0.352 | 0.284 |
| 0.16 | 0.211 | 0.143 |
| 0.00 | 0.068 | 0.000 |
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant TIMP1 and the recovery rates were calculated by comparing the measured value to the expected amount of TIMP1 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 86-99% | 92% |
| EDTA plasma(n=5) | 79-93% | 86% |
| Heparin plasma(n=5) | 90-105% | 97% |
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 83-95% | 88-102% | 85-97% | 80-92% |
| EDTA plasma(n=5) | 98-107% | 93-102% | 80-93% | 91-100% |
| Heparin plasma(n=5) | 88-95% | 98-107% | 90-99% | 88-97% |
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