Lablisa® Human APOB100(Apolipoprotein B100) ELISA Kit
Product Information
- Description
- More Infomation
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOB100. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOB100. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOB100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOB100 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Human APOB100(Apolipoprotein B100) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | Apo-B100 |
| Assay Type: | Sandwich |
| Sensitivity: | 9.15 ng/mL |
| Standard: | 1500 ng/mL |
| Detection Range: | 23.44-1500 ng/mL |
| Sample Type: | Serum, plasma and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 1500.00 | 2.075 | 1.967 |
| 750.00 | 1.647 | 1.539 |
| 375.00 | 1.185 | 1.077 |
| 187.50 | 0.796 | 0.688 |
| 93.75 | 0.538 | 0.430 |
| 46.88 | 0.364 | 0.256 |
| 23.44 | 0.216 | 0.108 |
| 0.00 | 0.108 | 0.000 |
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant APOB100 and the recovery rates were calculated by comparing the measured value to the expected amount of APOB100 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 89-101% | 95% |
| EDTA plasma(n=5) | 82-95% | 88% |
| Heparin plasma(n=5) | 80-94% | 87% |
Linearity
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 81-93% | 86-98% | 91-103% | 82-95% |
| EDTA plasma(n=5) | 87-95% | 85-98% | 82-97% | 92-101% |
| Heparin plasma(n=5) | 83-92% | 91-96% | 85-93% | 95-101% |
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