Tri-color Prestained Protein Ladder
Product Information
- Description
- More Infomation
The Tri-color Prestained Protein Ladder is a combination of 10
pre-stained proteins with molecular weights from 6.5 to 270 kDa. The 10
recombinant proteins are covalently coupled with blue chromophore, while 2
orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as
reference bands. The Tri-color Prestained Protein Ladder keeps track of the
size and separation ofproteins during SDS-polyacrylamide gel electrophoresis,
approximating protein size and validating Western transfer efficiency on PVDF,
nylon, or nitrocellulose membranes.
STORAGE BUFFER
62.5 mM Tris-H3PO4 (pH 7.5 at 25 °C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol.
REACTION SETUP
3μl or 5μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5μl per well for general Western transferring.
IMPORTANT NOTES
The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data
should be considered for a more accurate adjustment.
STORAGE CONDITION:
· Stable for up to 24 months at -20°C.
· Stable for up to 3 months at 4°C.