Lablisa® Human IFABP/FABP2(Intestinal Fatty Acid Binding Protein) ELISA Kit
Product Information
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFABP/FABP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFABP/FABP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFABP/FABP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFABP/FABP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Human IFABP/FABP2(Intestinal Fatty Acid Binding Protein) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein; FABP2; Fatty Acid Binding Protein 2, Intestinal |
| Assay Type: | Sandwich |
| Sensitivity: | 0.061 ng/mL |
| Standard: | 10 ng/mL |
| Detection Range: | 0.16-10 ng/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Metabolic pathway;Tumor immunity;Endocrinology;Cardiovascular biology; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 10.00 | 2.035 | 1.928 |
| 5.00 | 1.593 | 1.486 |
| 2.50 | 1.183 | 1.076 |
| 1.25 | 0.752 | 0.645 |
| 0.63 | 0.546 | 0.439 |
| 0.32 | 0.354 | 0.247 |
| 0.16 | 0.232 | 0.125 |
| 0.00 | 0.107 | 0.000 |
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant IFABP/FABP2 and the recovery rates were calculated by comparing the measured value to the expected amount of IFABP/FABP2 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 82-95% | 88% |
| EDTA plasma(n=5) | 80-95% | 87% |
| Heparin plasma(n=5) | 86-99% | 92% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFABP/FABP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 87-98% | 92-105% | 95-107% | 82-96% |
| EDTA plasma(n=5) | 81-94% | 93-107% | 93-102% | 91-105% |
| Heparin plasma(n=5) | 89-98% | 81-94% | 96-105% | 92-103% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
