Lablisa® Human IFABP/FABP2(Intestinal Fatty Acid Binding Protein) ELISA Kit

Product Information

Lablisa® Human IFABP/FABP2(Intestinal Fatty Acid Binding Protein) ELISA Kit

Catalog No : LAB1264 | Pack Size : 48T & 96T 

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFABP/FABP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFABP/FABP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFABP/FABP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFABP/FABP2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Product name:Human IFABP/FABP2(Intestinal Fatty Acid Binding Protein) ELISA Kit
Alternative Names:FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein; FABP2; Fatty Acid Binding Protein 2, Intestinal
Assay Type:Sandwich
Sensitivity:0.061 ng/mL
Standard:10 ng/mL
Detection Range:0.16-10 ng/mL
Sample Type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:3.5h
Research Area:Metabolic pathway;Tumor immunity;Endocrinology;Cardiovascular biology;

Standard curve

Concentration (ng/mL)ODCorrected OD


Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.


Matrices listed below were spiked with certain level of recombinant IFABP/FABP2 and the recovery rates were calculated by comparing the measured value to the expected amount of IFABP/FABP2 in samples.

MatrixRecovery rangeAverage
EDTA plasma(n=5)80-95%87%
Heparin plasma(n=5)86-99%92%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFABP/FABP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

EDTA plasma(n=5)81-94%93-107%93-102%91-105%
Heparin plasma(n=5)89-98%81-94%96-105%92-103%

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