Lablisa® E2(Estradiol) ELISA Kit
Product Information
- Description
- More Infomation
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E2. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to E2. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product name: | Lablisa® E2(Estradiol) ELISA Kit |
| Reactivity: | Universal |
| Alternative Names: | Oestradiol; E2; 17β-Estradiol; Estra-1,3,5(10)-triene-3,17β-diol |
| Assay Type: | Competitive |
| Sensitivity: | 7.5 pg/mL |
| Standard: | 800pg/mL |
| Range: | 12.5-800 pg/mL |
| Sample Type: | Serum, Plasma, Other biological fluids,Sample volume: 50μL |
| Assay Length: | 1.5h |
| Research Area: | Signaling transduction；Cancer；Metabolism |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 800.00 | 0.185 |
| 400.00 | 0.345 |
| 200.00 | 0.624 |
| 100.00 | 0.968 |
| 50.00 | 1.385 |
| 25.00 | 1.648 |
| 12.50 | 1.825 |
| 0.00 | 2.061 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 83-97% | 90% |
| EDTA plasma(n=8) | 80-97% | 88% |
| Cell culture media (n=8) | 85-101% | 93% |
Linearity
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 85-94% | 87-96% | 92-105% | 89-102% |
| EDTA plasma(n=5) | 85-102% | 85-102% | 80-92% | 87-103% |
| Cell culture media (n=5) | 89-98% | 80-97% | 96-104% | 85-93% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
