Lablisa® cAMP(Cyclic Adenosine Monophosphate) ELISA Kit
Product Information
- Description
- More Infomation
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cAMP. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to cAMP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of cAMP in the samples is then determined by comparing the OD of the samples to the standard curve.
Product name: | Lablisa® cAMP(Cyclic Adenosine Monophosphate) ELISA Kit |
| Reactivity: | Universal |
| Alternative Names: | c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate |
| Assay Type: | Competitive |
| Sensitivity: | 92.5 pg/mL |
| Standard: | 20000 pg/mL |
| Range: | 312.5-20000 pg/mL |
| Sample Type: | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids |
| Assay Length: | 1.5h |
| Research Area: | Signal transduction;Metabolic pathway; |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 20000.00 | 0.195 |
| 10000.00 | 0.331 |
| 5000.00 | 0.546 |
| 2500.00 | 0.880 |
| 1250.00 | 1.308 |
| 625.00 | 1.700 |
| 312.50 | 2.076 |
| 0.00 | 2.516 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 80-92% | 86% |
| EDTA plasma(n=8) | 90-105% | 97% |
| Cell culture media (n=8) | 88-102% | 95% |
Linearity
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 85-94% | 87-99% | 85-101% | 80-90% |
| EDTA plasma(n=5) | 86-97% | 86-97% | 88-95% | 92-101% |
| Cell culture media (n=5) | 87-98% | 85-92% | 80-96% | 89-100% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
