Lablisa® Rat AOPP(Advanced Oxidation Protein Products) ELISA Kit
Product Information
- Description
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This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AOPP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AOPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AOPP in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Rat AOPP(Advanced Oxidation Protein Products) ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | AOPP |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 49.7 pg/mL |
| Standard: | 10000 pg/mL |
| Detection Range: | 156.25-10000 pg/mL |
| Sample Type: | Serum, plasma and other biological fluids |
| Assay Length: | 2h |
| Research Area: | Tumor immunity |
Standard curve
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 10000.00 | 0.197 | |
| 5000.00 | 0.352 | |
| 2500.00 | 0.583 | |
| 1250.00 | 0.833 | |
| 625.00 | 0.977 | |
| 312.50 | 1.201 | |
| 156.25 | 1.715 | |
| 0.00 | 2.101 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant AOPP and the recovery rates were calculated by comparing the measured value to the expected amount of AOPP in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 92-102% | 97% |
| EDTA plasma(n=5) | 81-95% | 88% |
| Heparin plasma(n=5) | 95-103% | 99% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AOPP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 85-92% | 79-96% | 89-100% | 85-96% |
| EDTA plasma(n=5) | 86-99% | 93-107% | 85-94% | 87-101% |
| Heparin plasma(n=5) | 78-102% | 83-96% | 79-93% | 88-97% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
