Lablisa® Dog Cys-C(Cystatin C) ELISA Kit
Product Information
- Description
- More Infomation
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Dog Cys-C(Cystatin C) ELISA Kit |
| Reactivity: | Dog |
| Alternative Names: | CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin |
| Assay Type: | Sandwich |
| Sensitivity: | 12.1 pg/mL |
| Standard: | 2000 pg/mL |
| Detection Range: | 31.25-2000 pg/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Tags & Cell Markers；Cell Biology；Stem Cells；Cardiovascular |
Standard curve
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 2000.00 | 2.132 | 2.045 |
| 1000.00 | 1.624 | 1.537 |
| 500.00 | 1.223 | 1.136 |
| 250.00 | 0.933 | 0.846 |
| 125.00 | 0.593 | 0.506 |
| 62.50 | 0.357 | 0.270 |
| 31.25 | 0.189 | 0.102 |
| 0.00 | 0.087 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 82-95% | 88% |
| EDTA plasma(n=5) | 94-107% | 100% |
| Heparin plasma(n=5) | 94-105% | 98% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 86-93% | 91-103% | 87-98% | 95-101% |
| EDTA plasma(n=5) | 93-99% | 87-101% | 85-95% | 85-96% |
| Heparin plasma(n=5) | 88-100% | 95-103% | 89-97% | 85-94% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
