Lablisa® FastTrack Human ADP/Acrp30(Adiponectin) ELISA Kit
Product Information
- Description
- More Infomation
This assay has high sensitivity and excellent specificity for detection of Human ADP/Acrp30. No significant cross-reactivity or interference between Human ADP/Acrp30 and analogues was observed.
| Product name: | Lablisa® FastTrack Human ADP/Acrp30(Adiponectin) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | GBP28, ACDC, APM1, ADPN, AdipoQ, ADIPQTL1 |
| Assay Type: | Sandwich |
| Sensitivity: | 3.47 pg/mL |
| Standard: | 2000 pg/mL |
| Range: | 31.25-2000 pg/mL |
| Sample Type: | Serum, Plasma and Other biological fluids；Sample Volume=50μL |
| Assay Length: | 1.5h |
| Research Area: | Cardiovascular，Signaling transduction，Cell biology，Neuroscience，Cancer，Immunology，Metabolism |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 2000.00 | 2.358 | 2.339 |
| 1000.00 | 1.786 | 1.767 |
| 500.00 | 1.014 | 0.996 |
| 250.50 | 0.60 | 0.582 |
| 125.00 | 0.263 | 0.245 |
| 62.50 | 0.151 | 0.133 |
| 31.25 | 0.076 | 0.058 |
| 0.00 | 0.018 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Human ADP/Acrp30 and the recovery rates were calculated by comparing the measured value to the expected amount of Human ADP/Acrp30 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 88-103% | 95% |
| EDTA plasma(n=8) | 92-106% | 99% |
| Heparin plasma(n=8) | 90-105% | 97% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human ADP/Acrp30 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=8) | 88-103% | 83-94% | 92-105% | 89-97% |
| EDTA plasma(n=8) | 95-106% | 95-106% | 88-103% | 81-98% |
| Heparin plasma(n=8) | 93-102% | 90-99% | 95-106% | 87-101% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
