Lablisa® Human CA15-3(Carbonhydrate Antigen 15-3) ELISA Kit
Product Information
- Description
- More Infomation
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA15-3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA15-3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA15-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA15-3 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® Human CA15-3(Carbonhydrate Antigen 15-3) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | CA15-3 |
| Assay Type: | Sandwich |
| Sensitivity: | 0.47 U/mL |
| Standard: | 100 U/mL |
| Range: | 1.57-100 U/mL |
| Sample Type: | serum, plasma and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Tumor immunity;Infection immunity; |
Standard curve
| Concentration (U/mL) | OD | Corrected OD |
|---|---|---|
| 100.00 | 2.126 | 2.040 |
| 50.00 | 1.613 | 1.527 |
| 25.00 | 1.226 | 1.140 |
| 12.50 | 0.826 | 0.740 |
| 6.25 | 0.576 | 0.490 |
| 3.13 | 0.373 | 0.287 |
| 1.57 | 0.135 | 0.049 |
| 0.00 | 0.086 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant CA15-3 and the recovery rates were calculated by comparing the measured value to the expected amount of CA15-3 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 81-95% | 88% |
| EDTA plasma(n=5) | 78-92% | 85% |
| Heparin plasma(n=5) | 88-106% | 97% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA15-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 87-96% | 91-101% | 87-98% | 89-103% |
| EDTA plasma(n=5) | 85-94% | 87-96% | 79-95% | 95-102% |
| Heparin plasma(n=5) | 85-92% | 79-96% | 87-103% | 90-99% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
