Lablisa® Rat NF-κBp105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit
Product Information
- Description
- More Infomation
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Rat NF-κBp105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B |
| Assay Type: | Sandwich |
| Sensitivity: | 0.114 ng/mL |
| Standard: | 20 ng/mL |
| Detection Range: | 0.32-20 ng/mL |
| Sample Type: | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Signal transduction; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 20.00 | 2.379 | 2.296 |
| 10.00 | 1.621 | 1.538 |
| 5.00 | 1.115 | 1.032 |
| 2.50 | 0.735 | 0.652 |
| 1.25 | 0.555 | 0.472 |
| 0.63 | 0.298 | 0.215 |
| 0.32 | 0.223 | 0.140 |
| 0.00 | 0.083 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant NF-κBp105 and the recovery rates were calculated by comparing the measured value to the expected amount of NF-κBp105 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 85-97% | 91% |
| EDTA plasma(n=5) | 81-93% | 87% |
| Heparin plasma(n=5) | 82-94% | 88% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NF-κBp105 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 87-102% | 82-91% | 86-93% | 81-94% |
| EDTA plasma(n=5) | 86-94% | 85-95% | 89-99% | 85-95% |
| Heparin plasma(n=5) | 89-97% | 85-94% | 79-96% | 85-99% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
