Lablisa® MF-Mouse TNNI3/cTn-I(Troponin I Type 3, Cardiac) ELISA Kit

Lablisa® MF-Mouse TNNI3/cTn-I(Troponin I Type 3, Cardiac) ELISA Kit

Product Information

Lablisa® MF-Mouse TNNI3/cTn-I(Troponin I Type 3, Cardiac) ELISA Kit

Catalog No : LAB9036 | Pack Size : 48T , 96T

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  • Description
  • More Infomation
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNNI3/cTn-I. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Mouse TNNI3/cTn-I. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNNI3/cTn-I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Mouse TNNI3/cTn-I. You can calculate the concentration of Mouse TNNI3/cTn-I in the samples by comparing the OD of the samples to the standard curve.
Product name:Lablisa® MF-Mouse TNNI3/cTn-I(Troponin I Type 3, Cardiac) ELISA Kit
Reactivity:Mouse
Alternative Names:TNNC1, cTnI, CMD1FF, CMD2A, CMH7, RCM1
Assay Type:Sandwich
Sensitivity:4.69 pg/mL
Standard:500pg/mL
Range:7.82-500pg/mL
Sample Type:Serum, Plasma and Tissue homogenate and Other biological samples；Sample volume：25μL
Assay Length:3.5h
Research Area:

Cardiovascular, Signaling transduction, Cancer, Developmental Biology, Stem Cells;


Standard curve



Concentration (ng/mL)ODCorrected OD

500.00

2.4862.435
250.001.6301.579
125.000.9640.913
62.500.4450.394
31.250.2360.185
15.630.1380.087
7.820.0860.035
0.000.0510.000


Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.


Recovery

The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

MatrixRecovery rangeAverage
serum(n=8)96-109%102%
EDTA plasma(n=8)80-93%86%
Cell culture media (n=8)80-97%88%


Linearity

Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Matrix1:21:41:81:16
serum(n=5)95-106%85-104%90-98%82-98%
EDTA plasma(n=5)80-97%86-92%81-93%89-97%
Cell culture media (n=5)85-94%97-106%92-101%82-90%


Manuals on the web are for your reference only, specifications are subject to the delivery manuals.

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