Lablisa® Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Product Information
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb |
| Assay Type: | Sandwich |
| Sensitivity: | 15 pg/mL |
| Standard: | 2500 pg/mL |
| Detection Range: | 39.07-2500 pg/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Endocrinology;Cardiovascular biology; |
Standard curve
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 2500.00 | 2.037 | 1.951 |
| 1250.00 | 1.572 | 1.486 |
| 625.00 | 1.216 | 1.130 |
| 312.50 | 0.921 | 0.835 |
| 156.25 | 0.563 | 0.477 |
| 78.13 | 0.403 | 0.317 |
| 39.07 | 0.256 | 0.170 |
| 0.00 | 0.086 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant NT-ProBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProBNP in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 90-103% | 96% |
| EDTA plasma(n=5) | 81-96% | 88% |
| Heparin plasma(n=5) | 78-94% | 86% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 82-94% | 95-102% | 87-103% | 88-95% |
| EDTA plasma(n=5) | 98-107% | 90-99% | 88-97% | 85-97% |
| Heparin plasma(n=5) | 81-94% | 87-105% | 79-95% | 96-105% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
