Lablisa® TXA2(Thromboxane A2) ELISA Kit
Product Information
- Description
- More Infomation
Product name: | Lablisa® TXA2(Thromboxane A2) ELISA Kit |
| Reactivity: | Universal |
| Alternative Names: | TXA2 |
| Assay Type: | Competitive |
| Sensitivity: | 4.39 pg/mL |
| Standard: | 1000 pg/mL |
| Range: | 15.63-1000 pg/mL |
| Sample Type: | Serum, Plasma and Other biological fluids |
| Assay Length: | 1.5h |
| Research Area: | Signal transduction;Infection immunity;Endocrinology;Hormone metabolism; |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 1000.00 | 0.289 |
| 500.00 | 0.389 |
| 250.00 | 0.583 |
| 125.00 | 0.844 |
| 62.50 | 1.139 |
| 31.25 | 1.534 |
| 15.63 | 1.848 |
| 0.00 | 2.512 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 84-103% | 96% |
| EDTA plasma(n=8) | 82-96% | 87% |
| Cell culture media (n=8) | 86-97% | 92% |
Linearity
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 95-102% | 98-106% | 87-98% | 91-102% |
| EDTA plasma(n=5) | 89-99% | 89-99% | 88-104% | 84-98% |
| Cell culture media (n=5) | 95-105% | 86-97% | 83-96% | 91-103% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
