Lablisa® FastTrack Human IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) ELISA Kit
Product Information
- Description
- More Infomation
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-2Ra/CD25, and the Human IL-2Ra/CD25 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL-2Ra/CD25. After TMB substrate solution is added, only those wells that contain Human IL-2Ra/CD25 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-2Ra/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® FastTrack Human IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha |
| Assay Type: | Sandwich |
| Sensitivity: | 37.66 pg/mL |
| Standard: | 6000 pg/mL |
| Detection Range: | 93.75-6000 pg/mL |
| Sample Type: | Serum, plasma |
| Assay Length: | 1.5h |
| Research Area: | Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity; |
Standard curve
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 6000.00 | 2.189 | 2.088 |
| 3000.00 | 1.582 | 1.481 |
| 1500.00 | 1.243 | 1.142 |
| 750.00 | 0.935 | 0.834 |
| 375.00 | 0.503 | 0.402 |
| 187.50 | 0.285 | 0.184 |
| 93.75 | 0.219 | 0.118 |
| 0.00 | 0.101 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant IL-2Ra/CD25 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-2Ra/CD25 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 81-97% | 89% |
| EDTA plasma(n=5) | 85-99% | 92% |
| Heparin plasma(n=5) | 90-105% | 97% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-2Ra/CD25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 79-96% | 85-99% | 79-93% | 89-97% |
| EDTA plasma(n=5) | 88-105% | 86-92% | 81-93% | 79-97% |
| Heparin plasma(n=5) | 85-102% | 87-96% | 92-101% | 89-104% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
