Lablisa® FastTrack E2(Estradiol) ELISA Kit
Product Information
- Description
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This assay has high sensitivity and excellent specificity for detection of E2. No significant cross-reactivity or interference between E2 and analogues was observed.
| Product name: | Lablisa® FastTrack E2(Estradiol) ELISA Kit |
| Reactivity: | Universal |
| Alternative Names: | Oestradiol; E2; 17β-Estradiol; Estra-1,3,5(10)-triene-3,17β-diol |
| Assay Type: | Competitive |
| Sensitivity: | 7.5 pg/mL |
| Standard: | 800 pg/mL |
| Range: | 12.5-800 pg/mL |
| Sample Type: | Serum, Plasma and Other biological fluids; Sample Volume=50μL |
| Assay Length: | 0.75H |
| Research Area: | Signaling transduction；Cancer；Metabolism |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 800 | 0.185 |
| 400 | 0.345 |
| 200 | 0.624 |
| 100 | 0.968 |
| 50 | 1.385 |
| 25 | 1.648 |
| 12.5 | 1.825 |
| 0.00 | 2.061 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant E2 and the recovery rates were calculated by comparing the measured value to the expected amount of E2 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 83-97% | 90% |
| EDTA plasma(n=8) | 80-97% | 88% |
| Heparin plasma(n=8) | 85-101% | 93% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=8) | 85-94% | 87-96% | 92-105% | 89-102% |
| EDTA plasma(n=8) | 85-102% | 85-102% | 80-92% | 87-103% |
| Heparin plasma(n=8) | 89-98% | 80-97% | 96-104% | 85-93% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
