Lablisa® FastTrack Human VEGF165(Vascular Endothelial Growth Factor 165) ELISA Kit
Product Information
- Description
- More Infomation
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGF165, and the Human VEGF165 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VEGF165. After TMB substrate solution is added, only those wells that contain Human VEGF165 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGF165 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® FastTrack Human VEGF165(Vascular Endothelial Growth Factor 165) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | VEGF165 |
| Assay Type: | Sandwich |
| Sensitivity: | 15 pg/mL |
| Standard: | 2000 pg/mL |
| Detection Range: | 31.25-2000 pg/mL |
| Sample Type: | Serum, plasma |
| Assay Length: | 1.5h |
| Research Area: | Cytokine;Tumor immunity; |
Standard curve
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 2000.00 | 2.126 | 2.033 |
| 1000.00 | 1.731 | 1.638 |
| 500.00 | 1.114 | 1.021 |
| 250.00 | 0.855 | 0.762 |
| 125.00 | 0.532 | 0.439 |
| 62.50 | 0.335 | 0.242 |
| 31.25 | 0.226 | 0.133 |
| 0.00 | 0.093 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant VEGF165 and the recovery rates were calculated by comparing the measured value to the expected amount of VEGF165 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 95-108% | 101% |
| EDTA plasma(n=5) | 83-97% | 90% |
| Heparin plasma(n=5) | 80-97% | 88% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VEGF165 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 85-97% | 87-96% | 85-96% | 85-95% |
| EDTA plasma(n=5) | 95-106% | 87-96% | 82-90% | 92-101% |
| Heparin plasma(n=5) | 85-92% | 95-103% | 97-105% | 93-102% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
