Lablisa® VB6(Vitamin B6) ELISA Kit
Product Information
- Description
- More Infomation
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VB6. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to VB6. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VB6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product name: | Lablisa® VB6(Vitamin B6) ELISA Kit |
| Reactivity: | Universal |
| Alternative Names: | Pyridoxine |
| Assay Type: | Competitive |
| Sensitivity: | 1.42 ng/mL |
| Standard: | 300 ng/mL |
| Range: | 4.69-300 ng/mL |
| Sample Type: | Serum, Plasma and Other biological fluids |
| Assay Length: | 1.5h |
| Research Area: | Nutrition metabolism;Hormone metabolism; |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 300.00 | 0.259 |
| 150.00 | 0.381 |
| 75.00 | 0.559 |
| 37.50 | 0.843 |
| 18.75 | 1.152 |
| 9.38 | 1.520 |
| 4.69 | 1.793 |
| 0.00 | 2.541 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 87-99% | 93% |
| EDTA plasma(n=8) | 90-104% | 97% |
| Cell culture media (n=8) | 80-97% | 88% |
Linearity
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 85-92% | 80-96% | 89-101% | 85-97% |
| EDTA plasma(n=5) | 86-100% | 86-100% | 93-108% | 80-101% |
| Cell culture media (n=5) | 87-96% | 96-105% | 95-103% | 95-103% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
