Lablisa® FastTrack Human Cys-C(CystatinC) ELISA Kit
Product Information
- Description
- More Infomation
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C, and the Human Cys-C standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human Cys-C. After TMB substrate solution is added, only those wells that contain Human Cys-C and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® FastTrack Human Cys-C(CystatinC) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin |
| Assay Type: | Sandwich |
| Sensitivity: | 25 pg/mL |
| Standard: | 5000 pg/mL |
| Detection Range: | 78.1-5000 pg/mL |
| Sample Type: | Serum, plasma |
| Assay Length: | 1.5h |
| Research Area: | Tags & Cell Markers;Cell Biology;Stem Cells;Cardiovascular |
Standard curve
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 5000.00 | 2.781 | 2.716 |
| 2500.00 | 1.701 | 1.637 |
| 1250.00 | 0.906 | 0.842 |
| 625.00 | 0.508 | 0.443 |
| 312.50 | 0.319 | 0.254 |
| 156.25 | 0.177 | 0.113 |
| 78.13 | 0.120 | 0.056 |
| 0.00 | 0.064 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Cys-C and the recovery rates were calculated by comparing the measured value to the expected amount of Cys-C in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 88-99% | 94% |
| EDTA plasma(n=5) | 102-110% | 104% |
| Heparin plasma(n=5) | 98-104% | 101% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cys-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 93-106% | 88-102% | 85-101% | 96-107% |
| EDTA plasma(n=5) | 90-103% | 94-108% | 90-106% | 98-105% |
| Heparin plasma(n=5) | 91-100% | 98-107% | 92-108% | 93-102% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
