Lablisa® Human 8-OHdG(DNA Damage) ELISA Kit
Product Information
- Description
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This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 8-OHdG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® Human 8-OHdG(DNA Damage) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | 8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 8-Hydroxydeoxyguanosine |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 26.29 pg/mL |
| Standard: | 6000 pg/mL |
| Detection Range: | 93.75-6000 pg/mL |
| Sample Type: | serum, plasma and other biological fluids |
| Assay Length: | 2h |
| Research Area: | Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology; |
Standard curve
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 6000.00 | 0.191 | |
| 3000.00 | 0.367 | |
| 1500.00 | 0.596 | |
| 750.00 | 0.861 | |
| 375.00 | 1.157 | |
| 187.50 | 1.585 | |
| 93.75 | 1.775 | |
| 0.00 | 2.123 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant 8-OHdG and the recovery rates were calculated by comparing the measured value to the expected amount of 8-OHdG in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 95-107% | 101% |
| EDTA plasma(n=5) | 80-93% | 86% |
| Heparin plasma(n=5) | 94-105% | 87% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-OHdG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 92-101% | 87-98% | 85-94% | 87-101% |
| EDTA plasma(n=5) | 86-94% | 93-102% | 87-96% | 92-105% |
| Heparin plasma(n=5) | 88-102% | 87-101% | 85-95% | 85-96% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
