Lablisa® FastTrack Rat ADP/Acrp30(Adiponectin) ELISA Kit
Product Information
- Description
- More Infomation
This assay has high sensitivity and excellent specificity for detection of Rat ADP/Acrp30. No significant cross-reactivity or interference between Rat ADP/Acrp30 and analogues was observed.
| Product name: | Lablisa® FastTrack Rat ADP/Acrp30(Adiponectin) ELISA Kit | |
| Reactivity: | Rat | |
| Alternative Names: | Acrp3, GBP28, ACDC, APM1, ADPN, AdipoQ, ADIPQTL1 | |
| Assay Type: | Sandwich | |
| Sensitivity: | 0.94 ng/mL | |
| Standard: | 100 ng/mL | |
| Range: | 1.57-100 ng/mL | |
| Sample Type: |
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| Assay Length: | 1.5H | |
| Research Area: |
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Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 100 | 2.361 | 2.311 |
| 50 | 1.559 | 1.509 |
| 25 | 0.956 | 0.906 |
| 12.5 | 0.524 | 0.474 |
| 6.25 | 0.269 | 0.219 |
| 3.13 | 0.154 | 0.104 |
| 1.57 | 0.093 | 0.043 |
| 0.00 | 0.05 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Rat ADP/Acrp30 and the recovery rates were calculated by comparing the measured value to the expected amount of Rat ADP/Acrp30 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 83-95% | 89% |
| EDTA plasma(n=8) | 90-105% | 97% |
| Heparin plasma(n=8) | 80-95% | 87% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rat ADP/Acrp30 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=8) | 87-103% | 80-92% | 86-99% | 84-97% |
| EDTA plasma(n=8) | 85-102% | 80-92% | 87-98% | 95-102% |
| Heparin plasma(n=8) | 80-97% | 96-104% | 85-93% | 89-97% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
