Lablisa® Human CA19-9(Carbohydrate Antigen 19-9) ELISA Kit
Product Information
- Description
- More Infomation
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CA19-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CA19-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CA19-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CA19-9 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Human CA19-9(Carbohydrate Antigen 19-9) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | sialylated Lewis(a)Antigen; Cancer Antigen 19-9 |
| Assay Type: | Sandwich |
| Sensitivity: | 0.28 U/mL |
| Standard: | 50 U/mL |
| Detection Range: | 0.79-50 U/mL |
| Sample Type: | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Assay Length: | 3.5h |
| Research Area: | Tumor immunity;Hepatology;Gastroenterology; |
Standard curve
| Concentration (U/mL) | OD | Corrected OD |
|---|---|---|
| 50.00 | 1.882 | 1.796 |
| 25.00 | 1.517 | 1.431 |
| 12.50 | 1.035 | 0.949 |
| 6.25 | 0.782 | 0.696 |
| 3.13 | 0.541 | 0.455 |
| 1.57 | 0.354 | 0.268 |
| 0.79 | 0.232 | 0.146 |
| 0.00 | 0.086 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant CA19-9 and the recovery rates were calculated by comparing the measured value to the expected amount of CA19-9 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 82-95% | 88% |
| EDTA plasma(n=5) | 90-103% | 96% |
| Heparin plasma(n=5) | 85-97% | 91% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA19-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 88-97% | 95-106% | 83-98% | 93-105% |
| EDTA plasma(n=5) | 88-101% | 90-98% | 92-106% | 85-97% |
| Heparin plasma(n=5) | 91-104% | 82-96% | 88-101% | 90-99% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
