Lablisa® Rat GROa/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit
Product Information
- Description
- More Infomation
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GROa/CXCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GROa/CXCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GROa/CXCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GROa/CXCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Rat GROa/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | CXCL1; GRO1; GROa; GRO-A; MGSA-A; NAP3; SCYB1; Chemokine C-X-C-Motif Ligand 1; Melanoma Growth Stimulating Activity Alpha; Fibroblast Secretory Protein; Neutrophil Activating Protein 3 |
| Assay Type: | Sandwich |
| Sensitivity: | 6.7 pg/mL |
| Standard: | 1000 pg/mL |
| Detection Range: | 15.63-1000 pg/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Cytokine;Tumor immunity;Infection immunity; |
Standard curve
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 1000.00 | 2.038 | 1.949 |
| 500.00 | 1.609 | 1.520 |
| 250.00 | 0.974 | 0.885 |
| 125.00 | 0.659 | 0.570 |
| 62.50 | 0.447 | 0.358 |
| 31.25 | 0.352 | 0.263 |
| 15.63 | 0.203 | 0.114 |
| 0.00 | 0.089 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant GROa/CXCL1 and the recovery rates were calculated by comparing the measured value to the expected amount of GROa/CXCL1 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 82-95% | 88% |
| EDTA plasma(n=5) | 81-95% | 88% |
| Heparin plasma(n=5) | 90-103% | 96% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GROa/CXCL1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 88-104% | 84-98% | 95-105% | 88-102% |
| EDTA plasma(n=5) | 78-92% | 83-96% | 95-107% | 87-102% |
| Heparin plasma(n=5) | 87-98% | 92-105% | 95-107% | 82-96% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
