Lablisa® FastTrack Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
Product Information
- Description
- More Infomation
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D antigen, and the Human 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® FastTrack Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | 25 Hydroxy Vitamin D; |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 7.7 ng/mL |
| Standard: | 5000 ng/mL |
| Detection Range: | 78.13-5000 ng/mL |
| Sample Type: | Serum, plasma |
| Assay Length: | 1.5h |
| Research Area: | Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 5000.00 | 0.221 | |
| 2500.00 | 0.411 | |
| 1250.00 | 0.711 | |
| 625.00 | 0.959 | |
| 312.50 | 1.253 | |
| 156.25 | 1.579 | |
| 78.13 | 1.761 | |
| 0.00 | 2.375 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant 25-OH-D and the recovery rates were calculated by comparing the measured value to the expected amount of 25-OH-D in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 78-93% | 85% |
| EDTA plasma(n=5) | 87-99% | 93% |
| Heparin plasma(n=5) | 87-95% | 91% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 25-OH-D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 87-98% | 92-104% | 82-96% | 95-102% |
| EDTA plasma(n=5) | 90-99% | 83-97% | 81-103% | 89-101% |
| Heparin plasma(n=5) | 88-98% | 87-103% | 89-97% | 85-94% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
