Lablisa® DHT(Dihydrotestosterone) ELISA Kit
Product Information
- Description
- More Infomation
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DHT. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to DHT. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHT in the samples is then determined by comparing the OD of the samples to the standard curve.
Product name: | Lablisa® DHT(Dihydrotestosterone) ELISA Kit |
| Reactivity: | Universal |
| Alternative Names: | 5α-DHT; 5α-Dihydrotestosterone; Androstanolone; Stanolone |
| Assay Type: | Competitive |
| Sensitivity: | 13.3 pg/mL |
| Standard: | 2500 pg/mL |
| Range: | 39.07-2500 pg/mL |
| Sample Type: | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids |
| Assay Length: | 1.5h |
| Research Area: | Endocrinology;Reproductive science;Hormone metabolism; |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 2500.00 | 0.160 |
| 1250.00 | 0.337 |
| 625.00 | 0.542 |
| 312.50 | 0.852 |
| 156.25 | 1.194 |
| 78.13 | 1.576 |
| 39.06 | 1.836 |
| 0.00 | 2.467 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 95-107% | 101% |
| EDTA plasma(n=8) | 81-95% | 88% |
| Cell culture media (n=8) | 88-96% | 92% |
Linearity
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 88-104% | 93-102% | 82-97% | 92-101% |
| EDTA plasma(n=5) | 92-104% | 92-104% | 93-99% | 97-103% |
| Cell culture media (n=5) | 82-93% | 80-92% | 85-96% | 93-102% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
