Lablisa® FastTrack Human IL-8(Interleukin 8) ELISA Kit
Product Information
- Description
- More Infomation
This assay has high sensitivity and excellent specificity for detection of Human IL-8. No significant cross-reactivity or interference between Human IL-8 and analogues was observed.
| Product name: | Lablisa® FastTrack Human IL-8(Interleukin 8) ELISA Kit | |
| Reactivity: | Human | |
| Alternative Names: |
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| Assay Type: | Sandwich | |
| Sensitivity: | 4.69 pg/mL | |
| Standard: | 500 pg/mL | |
| Range: | 7.82-500 pg/mL | |
| Sample Type: |
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| Assay Length: | 1.5h | |
| Research Area: |
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Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 500 | 2.497 | 2.435 |
| 250 | 1.641 | 1.579 |
| 125 | 0.975 | 0.913 |
| 62.5 | 0.456 | 0.394 |
| 31.25 | 0.247 | 0185 |
| 15.63 | 0.149 | 0.087 |
| 7.82 | 0.097 | 0.035 |
| 0.00 | 0.062 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Human IL-8 and the recovery rates were calculated by comparing the measured value to the expected amount of Human IL-8 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 85-103% | 94% |
| EDTA plasma(n=8) | 95-105% | 100% |
| Heparin plasma(n=8) | 93-101% | 97% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human IL-8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=8) | 89-97% | 85-94% | 87-96% | 85-97% |
| EDTA plasma(n=8) | 85-94% | 85-94% | 87-96% | 92-101% |
| Heparin plasma(n=8) | 82-98% | 87-91% | 85-93% | 89-97% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
