Lablisa® Human Anti-GM1(Anti-Ganglioside Antibody) ELISA Kit
Product Information
- Description
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-GM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-GM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-GM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-GM1 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Human Anti-GM1(Anti-Ganglioside Antibody) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | anti-ganglioside (GM1) antibody (IgG) |
| Assay Type: | Sandwich |
| Sensitivity: | 0.57 ng/mL |
| Standard: | 80 ng/mL |
| Detection Range: | 1.25-80 ng/mL |
| Sample Type: | Serum, plasma, tissue homogenates and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Immune molecule |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 80.00 | 2.153 | 2.051 |
| 40.00 | 1.755 | 1.653 |
| 20.00 | 1.223 | 1.121 |
| 10.00 | 0.933 | 0.831 |
| 5.00 | 0.553 | 0.451 |
| 2.50 | 0.372 | 0.270 |
| 1.25 | 0.251 | 0.149 |
| 0.00 | 0.102 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Anti-GM1 and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-GM1 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 90-105% | 97% |
| EDTA plasma(n=5) | 80-97% | 88% |
| Heparin plasma(n=5) | 78-92% | 85% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-GM1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 88-99% | 86-92% | 89-103% | 97-105% |
| EDTA plasma(n=5) | 95-104% | 87-96% | 82-96% | 89-103% |
| Heparin plasma(n=5) | 85-92% | 89-96% | 90-98% | 87-98% |
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