Lablisa® MF-Mouse MMP-8(Matrix Metalloproteinase 8) ELISA Kit
Product Information
- Description
- More Infomation
| Product name: | Lablisa® MF-Mouse MMP-8(Matrix Metalloproteinase 8) ELISA Kit |
| Reactivity: | Mouse |
| Alternative Names: | MMP8, CLG1, HNC, PMNL-CL, Neutrophil Collagenase |
| Assay Type: | Sandwich |
| Sensitivity: | 0.19 ng/mL |
| Standard: | 20ng/mL |
| Range: | 0.32-20ng/mL |
| Sample Type: | Serum, Plasma and Tissue homogenate and Other biological samples；Sample volume：25μL |
| Assay Length: | 3.5h |
| Research Area: | Signaling transduction, Cancer; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
20.00 | 2.330 | 2.284 |
| 10.00 | 1.536 | 1.490 |
| 5.00 | 0.905 | 0.859 |
| 2.50 | 0.422 | 0.376 |
| 1.25 | 0.243 | 0.197 |
| 0.63 | 0.143 | 0.097 |
| 0.32 | 0.096 | 0.050 |
| 0.00 | 0.046 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 81-95% | 88% |
| EDTA plasma(n=8) | 83-95% | 89% |
| Cell culture media (n=8) | 87-97% | 90% |
Linearity
- Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 86-101% | 86-101% | 87-96% | 92-105% |
| EDTA plasma(n=5) | 89-102% | 92-101% | 88-102% | 87-102% |
| Cell culture media (n=5) | 85-96% | 87-101% | 89-102% | 85-98% |
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