Lablisa® KYNA(Kynurenic Acid) ELISA Kit
Product Information
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This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with KYNA. During the reaction, KYNA in the sample or standard competes with a fixed amount of KYNA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to KYNA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of KYNA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Product name: | Lablisa® KYNA(Kynurenic Acid) ELISA Kit |
| Reactivity: | Universal |
| Alternative Names: | Kinurenic acid; Kynuronic acid; Kuinurenic acid; Transtorine |
| Assay Type: | Competitive |
| Sensitivity: | 0.94 ng/mL |
| Standard: | 100ng/mL |
| Range: | 1.57-100ng/mL |
| Sample Type: | Serum, Urine, Tissue homogenates, Sample volume: 50μL |
| Assay Length: | 1.5h |
| Research Area: | Metabolism |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 100.00 | 0.258 |
| 50.00 | 0.358 |
| 25.00 | 0.552 |
| 12.50 | 0.813 |
| 6.25 | 1.108 |
| 3.13 | 1.503 |
| 1.56 | 1.817 |
| 0.00 | 2.481 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 80-95% | 87% |
| EDTA plasma(n=8) | 86-99% | 92% |
| Cell culture media (n=8) | 82-97% | 89% |
Linearity
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 88-97% | 93-103% | 94-106% | 91-101% |
| EDTA plasma(n=5) | 88-101% | 88-101% | 93-106% | 85-95% |
| Cell culture media (n=5) | 92-104% | 82-96% | 95-102% | 87-98% |
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