Lablisa® FastTrack Human VEGF-D(Vascular Endothelial Growth Factor D) ELISA Kit
Product Information
- Description
- More Infomation
This assay has high sensitivity and excellent specificity for detection of Human VEGF-D. No significant cross-reactivity or interference between Human VEGF-D and analogues was observed.
| Product name: | Lablisa® FastTrack Human VEGF-D(Vascular Endothelial Growth Factor D) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | FIGF, VEGF-D, VEGFD, C-fos induced growth factor |
| Assay Type: | Sandwich |
| Sensitivity: | 37.5 pg/mL |
| Standard: | 4000 pg/mL |
| Range: | 62.5-4000 pg/mL |
| Sample Type: | Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL |
| Assay Length: | 1.5H |
| Research Area: | Metabolic pathway; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 4000 | 2.378 | 2.334 |
| 2000 | 1.538 | 1.494 |
| 1000 | 0.864 | 0.82 |
| 500 | 0.464 | 0.42 |
| 250 | 0.244 | 0.2 |
| 125 | 0.141 | 0.097 |
| 62.5 | 0.094 | 0.05 |
| 0.00 | 0.044 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Human VEGF-D and the recovery rates were calculated by comparing the measured value to the expected amount of Human VEGF-D in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 85-99% | 92% |
| EDTA plasma(n=8) | 83-95% | 89% |
| Heparin plasma(n=8) | 96-107% | 102% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human VEGF-D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=8) | 85-104% | 87-106% | 83-92% | 82-90% |
| EDTA plasma(n=8) | 80-94% | 90-99% | 87-103% | 83-96% |
| Heparin plasma(n=8) | 88-102% | 96-107% | 89-103% | 80-95% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
