Lablisa® FastTrack Rat fT3(Free Triiodothyronine) ELISA Kit
Product Information
- Description
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This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat fT3 antigen, and the Rat fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® FastTrack Rat fT3(Free Triiodothyronine) ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | 3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 0.31 ng/mL |
| Standard: | 20 ng/mL |
| Detection Range: | 0.32-20 ng/mL |
| Sample Type: | Serum, plasma |
| Assay Length: | 1.5h |
| Research Area: | Endocrinology;Autoimmunity; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 20.00 | 0.235 | |
| 10.00 | 0.479 | |
| 5.00 | 0.722 | |
| 2.50 | 0.997 | |
| 1.25 | 1.236 | |
| 0.63 | 1.596 | |
| 0.32 | 1.861 | |
| 0.00 | 2.232 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant fT3 and the recovery rates were calculated by comparing the measured value to the expected amount of fT3 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 92-105% | 98% |
| EDTA plasma(n=5) | 89-103% | 96% |
| Heparin plasma(n=5) | 88-102% | 95% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of fT3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 85-94% | 90-99% | 95-102% | 82-90% |
| EDTA plasma(n=5) | 85-92% | 83-96% | 93-102% | 87-98% |
| Heparin plasma(n=5) | 87-90% | 87-98% | 85-96% | 85-95% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
