Lablisa® Human BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit
Product Information
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Human BMG/b2-MG(Beta-2-Microglobulin) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin |
| Assay Type: | Sandwich |
| Sensitivity: | 0.16 ng/mL |
| Standard: | 25 ng/mL |
| Detection Range: | 0.4-25 ng/mL |
| Sample Type: | serum, plasma, urine and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Infection immunity;Kidney biomarker; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 25.00 | 2.201 | 2.117 |
| 12.50 | 1.593 | 1.509 |
| 6.25 | 1.121 | 1.037 |
| 3.13 | 0.892 | 0.808 |
| 1.57 | 0.547 | 0.463 |
| 0.79 | 0.345 | 0.261 |
| 0.40 | 0.265 | 0.181 |
| 0.00 | 0.084 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant BMG/b2-MG and the recovery rates were calculated by comparing the measured value to the expected amount of BMG/b2-MG in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 78-91% | 84% |
| EDTA plasma(n=5) | 88-101% | 94% |
| Heparin plasma(n=5) | 82-95% | 88% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BMG/b2-MG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 88-105% | 79-93% | 87-98% | 85-96% |
| EDTA plasma(n=5) | 88-97% | 97-105% | 81-93% | 89-102% |
| Heparin plasma(n=5) | 88-102% | 81-96% | 86-93% | 82-95% |
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