Lablisa® FastTrack Human ICAM-1/CD54(intercellular adhesion molecule 1) ELISA Kit
Product Information
- Description
- More Infomation
This assay has high sensitivity and excellent specificity for detection of Human ICAM-1/CD54. No significant cross-reactivity or interference between Human ICAM-1/CD54 and analogues was observed.
| Product name: | Lablisa® FastTrack Human ICAM-1/CD54(intercellular adhesion molecule 1) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | ICAM1, BB2, CD54, P3.58, intercellular adhesion molecule 1 |
| Assay Type: | Sandwich |
| Sensitivity: | 0.19 ng/mL |
| Standard: | 20 ng/mL |
| Range: | 0.32-20 ng/mL |
| Sample Type: | Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL |
| Assay Length: | 1.5H |
| Research Area: | Signal transduction; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 20 | 2.346 | 2.284 |
| 10 | 1.552 | 1.49 |
| 5 | 0.921 | 0.859 |
| 2.5 | 0.438 | 0.376 |
| 1.25 | 0.259 | 0.197 |
| 0.63 | 0.159 | 0.097 |
| 0.32 | 0.112 | 0.05 |
| 0.00 | 0.062 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Human ICAM-1/CD54 and the recovery rates were calculated by comparing the measured value to the expected amount of Human ICAM-1/CD54 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 96-109% | 102% |
| EDTA plasma(n=8) | 81-95% | 88% |
| Heparin plasma(n=8) | 85-97% | 91% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human ICAM-1/CD54 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=8) | 86-97% | 83-96% | 91-103% | 96-105% |
| EDTA plasma(n=8) | 95-102% | 95-102% | 98-106% | 87-98% |
| Heparin plasma(n=8) | 91-102% | 89-99% | 88-104% | 84-98% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
