Lablisa® FastTrack Rat BMG/β2-MG(Beta-2-Microglobulin) ELISA Kit
Product Information
- Description
- More Infomation
This assay has high sensitivity and excellent specificity for detection of Rat BMG/β2-MG. No significant cross-reactivity or interference between Rat BMG/β2-MG and analogues was observed.
| Product name: | Lablisa® FastTrack Rat BMG/β2-MG(Beta-2-Microglobulin) ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | B2MG, B2M |
| Assay Type: | Sandwich |
| Sensitivity: | 1.57 pg/mL |
| Standard: | 1000 pg/mL |
| Range: | 15.63-1000 pg/mL |
| Sample Type: | Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL |
| Assay Length: | 1.5H |
| Research Area: | Enzyme & Kinase; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 1000 | 2.33 | 2.284 |
| 500 | 1.536 | 1.49 |
| 250 | 0.905 | 0.859 |
| 125 | 0.422 | 0.376 |
| 62.5 | 0.243 | 0.197 |
| 31.25 | 0.143 | 0.097 |
| 15.63 | 0.096 | 0.05 |
| 0.00 | 0.046 | 00 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Rat BMG/β2-MG and the recovery rates were calculated by comparing the measured value to the expected amount of Rat BMG/β2-MG in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 85-97% | 91% |
| EDTA plasma(n=8) | 85-97% | 91% |
| Heparin plasma(n=8) | 83-99% | 95% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rat BMG/β2-MG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=8) | 95-103% | 80-96% | 93-106% | 87-98% |
| EDTA plasma(n=8) | 88-104% | 88-104% | 86-102% | 80-93% |
| Heparin plasma(n=8) | 89-97% | 85-94% | 87-101% | 82-95% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
