Lablisa® LPS(Lipopolysaccharides) ELISA Kit
Product Information
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Product name: | Lablisa® LPS(Lipopolysaccharides) ELISA Kit |
| Reactivity: | Universal |
| Alternative Names: | Lipopolysaccharides |
| Assay Type: | Competitive |
| Sensitivity: | 0.94 ng/mL |
| Standard: | 100 ng/mL |
| Range: | 1.57-100ng/mL |
| Sample Type: | Serum, Plasma, Tissue homogenate and Other biological samples |
| Assay Length: | 1.5h |
| Research Area: | Immunology |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 100 | 0.267 |
| 50 | 0.367 |
| 25 | 0.561 |
| 12.5 | 0.822 |
| 6.25 | 1.117 |
| 3.13 | 1.512 |
| 1.57 | 1.826 |
| 0.00 | 2.49 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant LPS and the recovery rates were calculated by comparing the measured value to the expected amount of LPS in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 87-99% | 93% |
| EDTA plasma(n=8) | 83-99% | 95% |
| Cell culture media (n=8) | 80-96% | 88% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LPS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 86-96% | 93-104% | 89-97% | 85-94% |
| EDTA plasma(n=5) | 85-92% | 85-92% | 97-103% | 87-98% |
| Cell culture media (n=5) | 95-102% | 98-103% | 86-92% | 87-93% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
