Lablisa® FastTrack Human MMP8(Matrix Metalloproteinase 8) ELISA Kit
Product Information
- Description
- More Infomation
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP-8, and the Human MMP-8 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MMP-8. After TMB substrate solution is added, only those wells that contain Human MMP-8 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP-8 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® FastTrack Human MMP8(Matrix Metalloproteinase 8) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | CLG2; HNC; PMNL-CL; Collagenase 2; Neutrophil Collagenase; PMNL collagenase |
| Assay Type: | Sandwich |
| Sensitivity: | 24 pg/mL |
| Standard: | 10 ng/mL |
| Detection Range: | 0.16-10 ng/mL |
| Sample Type: | Serum, plasma |
| Assay Length: | 1.5h |
| Research Area: | Enzyme & Kinase;Infection immunity;Cardiovascular biology; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 10.00 | 2.231 | 2.127 |
| 5.00 | 1.697 | 1.593 |
| 2.50 | 1.069 | 0.965 |
| 1.25 | 0.752 | 0.648 |
| 0.63 | 0.451 | 0.347 |
| 0.32 | 0.352 | 0.248 |
| 0.16 | 0.228 | 0.124 |
| 0.00 | 0.104 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant MMP-8 and the recovery rates were calculated by comparing the measured value to the expected amount of MMP-8 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 87-99% | 93% |
| EDTA plasma(n=5) | 85-97% | 91% |
| Heparin plasma(n=5) | 90-103% | 96% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MMP-8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 85-92% | 79-96% | 92-101% | 87-98% |
| EDTA plasma(n=5) | 92-105% | 82-92% | 86-94% | 81-95% |
| Heparin plasma(n=5) | 86-93% | 91-104% | 87-98% | 95-99% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
