Lablisa® VA(Vitamin A) ELISA Kit
Product Information
- Description
- More Infomation
This assay employs the competitive inhibition enzyme immuno-assay technique. The microtiter plate provided in this kit has been pre-coated with VA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VA. Next, Avid in conjugated to Horseradish Peroxidase (HRP) is added to each micro-plate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectro photo metrically at a wavelength of 450nm ± 10nm. The concentration of VA in the samples is then determined by comparing the OD of the samples to the standard curve.
Product name: | Lablisa® VA(Vitamin A) ELISA Kit |
| Reactivity: | Universal |
| Alternative Names: | VA(Vitamin A) |
| Assay Type: | Competitive |
| Sensitivity: | 9.38 ng/mL |
| Standard: | 1000 ng/mL |
| Range: | 15.63-1000 ng/mL |
| Sample Type: | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids |
| Assay Length: | 1.5h |
| Research Area: | Metabolic pathway;Endocrinology;Hormone metabolism; |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 1000.00 | 0.264 |
| 500.00 | 0.364 |
| 250.00 | 0.558 |
| 125.00 | 0.819 |
| 62.50 | 1.114 |
| 31.25 | 1.509 |
| 15.63 | 1.823 |
| 0.00 | 2.487 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 89-103% | 96% |
| EDTA plasma(n=8) | 83-97% | 90% |
| Cell culture media (n=8) | 86-99% | 92% |
Linearity
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 86-93% | 93-101% | 85-98% | 90-101% |
| EDTA plasma(n=5) | 92-101% | 92-101% | 87-98% | 85-92% |
| Cell culture media (n=5) | 80-96% | 95-103% | 80-96% | 93-106% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
