Lablisa® FastTrack Human VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit
Product Information
- Description
- More Infomation
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VE-Cadherin, and the Human VE-Cadherin standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human VE-Cadherin. After TMB substrate solution is added, only those wells that contain Human VE-Cadherin and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® FastTrack Human VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin,Vascular Endothelial; CDH5; Cadherin 5 |
| Assay Type: | Sandwich |
| Sensitivity: | 32.1 ng/mL |
| Standard: | 10 μg/mL |
| Detection Range: | 0.16-10 μg/mL |
| Sample Type: | Serum, plasma |
| Assay Length: | 1.5h |
| Research Area: | CD & Adhesion molecule;Tumor immunity;Infection immunity; |
Standard curve
| Concentration (μg/mL) | OD | Corrected OD |
|---|---|---|
| 10.00 | 2.075 | 1.987 |
| 5.00 | 1.608 | 1.520 |
| 2.50 | 1.205 | 1.117 |
| 1.25 | 0.852 | 0.764 |
| 0.63 | 0.514 | 0.426 |
| 0.32 | 0.317 | 0.229 |
| 0.16 | 0.252 | 0.164 |
| 0.00 | 0.088 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant VE-Cadherin and the recovery rates were calculated by comparing the measured value to the expected amount of VE-Cadherin in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 85-97% | 91% |
| EDTA plasma(n=5) | 80-95% | 87% |
| Heparin plasma(n=5) | 89-103% | 96% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VE-Cadherin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 87-98% | 92-105% | 95-107% | 82-96% |
| EDTA plasma(n=5) | 88-97% | 86-92% | 94-103% | 89-97% |
| Heparin plasma(n=5) | 96-105% | 87-96% | 90-98% | 92-101% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
