Lablisa® FastTrack Biotin Quantitative Determination ELISA Kit
Product Information
- Description
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This assay has high sensitivity and excellent specificity for detection of Biotin. No significant cross-reactivity or interference between Biotin and analogues was observed.
| Product name: | Lablisa® FastTrack Biotin Quantitative Determination ELISA Kit |
| Reactivity: | Universal |
| Alternative Names: | Biotin |
| Assay Type: | Competitive |
| Sensitivity: | 5.35 pg/mL |
| Standard: | 500 pg/mL |
| Range: | 7.81-500 pg/mL |
| Sample Type: | Serum, Plasma and Other biological fluids; Sample Volume=50μL |
| Assay Length: | 0.75H |
| Research Area: | Signaling transduction；Cancer；Immunology；Metabolism |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 500 | 0.254 |
| 250 | 0.376 |
| 125 | 0.561 |
| 62.5 | 0.807 |
| 31.25 | 1.089 |
| 15.63 | 1.382 |
| 7.81 | 1.658 |
| 0.00 | 2.445 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Biotin and the recovery rates were calculated by comparing the measured value to the expected amount of Biotin in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 90-105% | 97% |
| EDTA plasma(n=8) | 80-95% | 87% |
| Heparin plasma(n=8) | 83-95% | 89% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Biotin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=8) | 80-91% | 89-103% | 82-95% | 86-99% |
| EDTA plasma(n=8) | 86-97% | 86-97% | 90-98% | 80-93% |
| Heparin plasma(n=8) | 92-105% | 87-98% | 85-92% | 80-96% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
