Lablisa® FastTrack Human NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Product Information
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP, and the Human NT-ProBNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NT-ProBNP. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® FastTrack Human NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | NT-Pro-BNP; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb |
| Assay Type: | Sandwich |
| Sensitivity: | 0.94 ng/mL |
| Standard: | 60 ng/mL |
| Detection Range: | 0.94-60 ng/mL |
| Sample Type: | Serum, plasma |
| Assay Length: | 1.5h |
| Research Area: | Endocrinology;Cardiovascular biology; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 60.00 | 2.207 | 2.106 |
| 30.00 | 1.643 | 1.542 |
| 15.00 | 1.251 | 1.150 |
| 7.50 | 0.847 | 0.746 |
| 3.75 | 0.625 | 0.524 |
| 1.88 | 0.301 | 0.200 |
| 0.94 | 0.288 | 0.187 |
| 0.00 | 0.101 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant NT-ProBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProBNP in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 90-103% | 96% |
| EDTA plasma(n=5) | 81-96% | 88% |
| Heparin plasma(n=5) | 78-94% | 86% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 89-98% | 83-103% | 98-105% | 92-101% |
| EDTA plasma(n=5) | 96-101% | 85-92% | 79-96% | 89-101% |
| Heparin plasma(n=5) | 93-105% | 85-97% | 90-99% | 82-101% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
