Lablisa® 3-NT(3-Nitrotyrosine) ELISA Kit
Product Information
- Description
- More Infomation
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 3-NT. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to 3-NT. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.
Product name: | Lablisa® 3-NT(3-Nitrotyrosine) ELISA Kit |
| Reactivity: | Universal |
| Alternative Names: | 3-Nitro-L-Tyrosine; 3-Nitrotyrosine; Nitrotyrosine |
| Assay Type: | Competitive |
| Sensitivity: | 1.35 ng/mL |
| Standard: | 300 ng/mL |
| Range: | 4.69-300 ng/mL |
| Sample Type: | Serum, Plasma and Other biological fluids |
| Assay Length: | 1.5h |
| Research Area: | Metabolic pathway;Infection immunity;Rheumatology; |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 300.00 | 0.235 |
| 150.00 | 0.357 |
| 75.00 | 0.535 |
| 37.50 | 0.819 |
| 18.75 | 1.128 |
| 9.38 | 1.496 |
| 4.69 | 1.769 |
| 0.00 | 2.517 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 89-103% | 96% |
| EDTA plasma(n=8) | 83-97% | 90% |
| Cell culture media (n=8) | 86-99% | 92% |
Linearity
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 86-93% | 93-101% | 85-98% | 90-101% |
| EDTA plasma(n=5) | 92-101% | 92-101% | 87-98% | 85-92% |
| Cell culture media (n=5) | 80-96% | 95-103% | 80-96% | 93-106% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
