Lablisa® FastTrack Human IFNg(Interferon Gamma) ELISA Kit
Product Information
- Description
- More Infomation
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFN-γ, and the Human IFN-γ standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IFN-γ. After TMB substrate solution is added, only those wells that contain Human IFN-γ and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFN-γ in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® FastTrack Human IFNg(Interferon Gamma) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | IFN gamma; IFN-G; IFG; IFI; INFr; IFN; Immune Interferon |
| Assay Type: | Sandwich |
| Sensitivity: | 15 pg/mL |
| Standard: | 1000 pg/mL |
| Detection Range: | 15.63-1000 pg/mL |
| Sample Type: | Serum, plasma, cell |
| Assay Length: | 1.5h |
| Research Area: | Cytokine;Infection immunity; |
Standard curve
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 1000.00 | 2.038 | 1.930 |
| 500.00 | 1.521 | 1.413 |
| 250.00 | 1.186 | 1.078 |
| 125.00 | 0.892 | 0.784 |
| 62.50 | 0.447 | 0.339 |
| 31.25 | 0.481 | 0.373 |
| 15.63 | 0.226 | 0.118 |
| 0.00 | 0.108 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant IFN-γ and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-γ in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 82-95% | 88% |
| EDTA plasma(n=5) | 80-95% | 87% |
| Heparin plasma(n=5) | 86-99% | 92% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-γ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 95-103% | 79-96% | 93-106% | 87-98% |
| EDTA plasma(n=5) | 86-93% | 93-101% | 85-98% | 90-101% |
| Heparin plasma(n=5) | 78-92% | 79-93% | 94-106% | 83-97% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
