Lablisa® Rat CRP(C Reactive Protein) ELISA Kit
Product Information
- Description
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® Rat CRP(C Reactive Protein) ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | C-RP; PTX1; Pentraxin-Related; Pentraxin 1 |
| Assay Type: | Sandwich |
| Sensitivity: | 6.1 pg/mL |
| Standard: | 1000 pg/mL |
| Detection Range: | 15.63-1000 pg/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology; |
Standard curve
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 1000.00 | 1.809 | 1.712 |
| 500.00 | 1.306 | 1.209 |
| 250.00 | 0.830 | 0.733 |
| 125.00 | 0.547 | 0.450 |
| 62.50 | 0.406 | 0.309 |
| 31.25 | 0.377 | 0.280 |
| 15.63 | 0.215 | 0.118 |
| 0.00 | 0.097 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant hs-CRP and the recovery rates were calculated by comparing the measured value to the expected amount of hs-CRP in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 84-97% | 90% |
| EDTA plasma(n=5) | 81-95% | 88% |
| Heparin plasma(n=5) | 90-105% | 97% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-CRP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 80-94% | 90-99% | 87-103% | 83-96% |
| EDTA plasma(n=5) | 88-102% | 96-107% | 89-103% | 79-95% |
| Heparin plasma(n=5) | 81-94% | 78-90% | 83-95% | 86-97% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
