Lablisa® FastTrack Human VEGF-C(Vascular Endothelial Growth Factor C) ELISA Kit
Product Information
- Description
- More Infomation
This assay has high sensitivity and excellent specificity for detection of Human VEGF-C. No significant cross-reactivity or interference between Human VEGF-C and analogues was observed.
| Product name: | Lablisa® FastTrack Human VEGF-C(Vascular Endothelial Growth Factor C) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | VEGFC, Flt4-L, VRP |
| Assay Type: | Sandwich |
| Sensitivity: | 65.63 pg/mL |
| Standard: | 7000 pg/mL |
| Range: | 109.38-7000 pg/mL |
| Sample Type: | Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL |
| Assay Length: | 1.5H |
| Research Area: | Cytokine;Infection immunity;Immune molecule; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 7000 | 2.684 | 2.615 |
| 3500 | 1.76 | 1.691 |
| 1750 | 0.952 | 0.883 |
| 875 | 0.425 | 0.356 |
| 437.5 | 0.214 | 0.145 |
| 218.75 | 0.1256 | 0.0566 |
| 109.38 | 0.0922 | 0.0232 |
| 0.00 | 0.069 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Human VEGF-C and the recovery rates were calculated by comparing the measured value to the expected amount of Human VEGF-C in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 83-98% | 90% |
| EDTA plasma(n=8) | 81-95% | 88% |
| Heparin plasma(n=8) | 88-102% | 95% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human VEGF-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=8) | 88-97% | 86-102% | 91-103% | 89-97% |
| EDTA plasma(n=8) | 80-101% | 87-96% | 96-105% | 95-103% |
| Heparin plasma(n=8) | 85-92% | 80-96% | 95-103% | 87-98% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
