Lablisa® 5-HT(5-Hydroxytryptamine) ELISA Kit
Product Information
- Description
- More Infomation
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 5-HT. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to 5-HT. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.
Product name: | Lablisa® 5-HT(5-Hydroxytryptamine) ELISA Kit |
| Reactivity: | Universal |
| Alternative Names: | 5HT; Serotonin; N-Methyl-Gamma |
| Assay Type: | Competitive |
| Sensitivity: | 3.97 ng/mL |
| Standard: | 900 ng/mL |
| Range: | 14.07-900 ng/mL |
| Sample Type: | Serum, Plasma, Tissue homogenates |
| Assay Length: | 1.5h |
| Research Area: | Signal transduction;Infection immunity;Neuro science;Gastroenterology; |
Standard curve
| Concentration (ng/mL) | OD |
|---|---|
| 900.00 | 0.423 |
| 450.00 | 0.539 |
| 225.00 | 0.735 |
| 112.50 | 1.020 |
| 56.25 | 1.358 |
| 28.13 | 1.673 |
| 14.06 | 1.909 |
| 0.00 | 2.428 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 87-95% | 91% |
| EDTA plasma(n=8) | 81-95% | 88% |
| Cell culture media (n=8) | 83-97% | 90% |
Linearity
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 85-94% | 90-99% | 82-101% | 91-102% |
| EDTA plasma(n=5) | 85-103% | 85-103% | 83-96% | 95-102% |
| Cell culture media (n=5) | 87-98% | 80-92% | 96-105% | 86-97% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
